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Related Experiment Video

Updated: Sep 23, 2025

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
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Asymmetric Vesicles Self-Assembled by Amphiphilic Sequence-Controlled Polymers.

Pei Huang1, Meiwei Qi1, Chuanshuang Chen1

  • 1School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai 200240, China.

ACS Macro Letters
|May 13, 2022
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Summary

Scientists created asymmetric vesicles using sequence-controlled polymers. These vesicles show preferential outer membrane placement of SU and inner membrane placement of TEO, enabling new functions.

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Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Cell membrane lipid asymmetry is crucial for physiological and immunological functions.
  • Synthetic asymmetric vesicles are inspired by cell membranes for advanced material applications.
  • Amphiphilic block copolymers are commonly used to prepare asymmetric vesicles.

Purpose of the Study:

  • To report the formation of asymmetric vesicles self-assembled by amphiphilic sequence-controlled polymers.
  • To develop a fluorescence titration method for determining the distribution of polymer segments.
  • To investigate the self-assembly behavior of polymers with specific hydrophilic segments (SU and TEO).

Main Methods:

  • Self-assembly of amphiphilic sequence-controlled polymers into vesicles.
  • Fluorescence titration using europium(III) ions (Eu3+) to quantify segment distribution.
  • Analysis of polymer structure and vesicle morphology.

Main Results:

  • Asymmetric vesicles were successfully formed from sequence-controlled polymers containing SU and TEO segments.
  • SU units were preferentially located on the outer membrane, while TEO units were on the inner membrane.
  • Electrostatic repulsion of SU and U-shaped backbone folding facilitated the asymmetric distribution.

Conclusions:

  • Sequence-controlled polymers offer a versatile platform for creating complex self-assembled structures.
  • The developed method efficiently determines the uneven distribution of polymer segments in vesicles.
  • This work provides a powerful toolbox for designing advanced self-assembled materials with tailored properties.