Two Sides of the Same Coin: Light as a Tool to Control and Map Microsphere Design

Herein, we establish the effect of intensity and wavelength on the size of microparticles formed via precipitation polymerization, employing photocrosslinkable prepolymers. Simultaneous measurement of backscattered laser irradiation enabled real-time tracking of particle growth and provides the ability to vary the LED intensity (λ_max = 415 nm) during various stages of particle growth. Critically, particle diameters can be controlled between 200 and 700 nm by varying the LED power from 73 to 0.63 mW, respectively. High intensities during the nucleation phase-spanning only the initial seconds-were found to dictate the particle diameter, irrespective of the energy used during the growth phase. Finally, a bathochromic shift was observed between the wavelength generating the highest rate of particle formation and the absorbance maxima of the photoactive group. We submit that these findings are broadly applicable in the continuously developing field of photoinitiated synthesis of polymer particles.