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3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
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Cell fiber-based 3D tissue array for drug response assay.

Midori Kato-Negishi1, Jun Sawayama2, Masahiro Kawahara1

  • 1Department of Bio-Analytical Chemistry, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo, 202-8585, Japan.

Scientific Reports
|May 13, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel cell fiber (CF) array method for 3D tissue drug screening. This technique overcomes spheroid limitations, enabling uniform, nutrient-rich 3D tissue models for sensitive drug response assays.

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Area of Science:

  • Biotechnology
  • Drug Discovery
  • Tissue Engineering

Background:

  • 3D tissue models are crucial for drug screening but face challenges like nutrient deprivation and hypoxia in spheroids.
  • Existing spheroid-based 3D arrays are limited to specific tissues and struggle with uniformity and cell viability.

Purpose of the Study:

  • To establish a reproducible and sensitive 3D tissue-based drug screening assay system.
  • To develop uniform 3D tissue arrays that prevent central cell death and overcome spheroid limitations.

Main Methods:

  • A novel method for arraying cell-encapsulated tube-like tissues (cell fibers, CFs) with diameters <150 μm was developed.
  • A device was utilized to fix, uniformly section, and transfer CF fragments to a 96-well plate.
  • Drug response assays were performed on CF fragment arrays from various cell types, including cancer cells and neural stem cells.

Main Results:

  • The cell fiber array method successfully prevented nutrient deprivation and hypoxia.
  • Arrays demonstrated drug response differences across various cell types and identified 3D tissue-specific drug responses.
  • The system proved effective for drug screening in diverse tissue types.

Conclusions:

  • The proposed cell fiber array system offers a versatile platform for sensitive and reproducible 3D drug screening.
  • This method overcomes key limitations of spheroid-based arrays, enabling broader application in drug discovery.
  • The technology facilitates the assessment of cell-type-specific and 3D-tissue-specific drug sensitivities.