Integrated Micro-Devices for a Lab-in-Organoid Technology Platform: Current Status and Future Perspectives

Area of Science:

• Neuroscience
• Biotechnology
• Bioelectronics

Background:

• Organoids, derived from adult stem cells (ASCs) and pluripotent stem cells (PSCs), offer complex 3D models.
• Conventional neurophysiological techniques struggle with chronic recordings and high-throughput screening in brain organoids.
• There is a need for advanced methods to monitor neural activity and facilitate drug discovery in organoid models.

Purpose of the Study:

• To introduce and develop Lab-in-Organoid (LIO) technology for in-tissue sensing and actuation within 3D cell aggregates.
• To create advanced 3D biological brain models with integrated artificial functionalities.
• To enable routine, label-free functional measurements and assay development for drug discovery.

Main Methods:

• Utilizing cell-autonomous self-organization of brain cells for self-aggregation.
• Integrating bioelectronic micro-scale devices within 3D cell aggregates.
• Investigating the impact of sinusoidal electromagnetic fields on neuronal spheroids for wireless data transmission.

Main Results:

• Demonstrated the feasibility of the Lab-in-Organoid (LIO) concept for creating functionalized 3D brain models.
• Successfully integrated wireless silicon micro-devices into neuronal spheroids.
• Assessed the effects of electromagnetic fields, crucial for wireless power and data transfer.

Conclusions:

• Lab-in-Organoid (LIO) technology provides a novel platform for in-tissue sensing and actuation in brain organoids.
• This technology enhances capabilities for chronic neural recordings and label-free functional measurements.
• LIO technology holds significant promise for advancing drug discovery and understanding brain development.