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Related Experiment Video

Updated: Sep 23, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

Identification of pathogenic bacteria in complex samples using a smartphone based fluorescence microscope.

Vilhelm Müller¹, José M Sousa², Hatice Ceylan Koydemir^3,4,5

¹Department of Biology and Biological Engineering, Chalmers University of Technology Gothenburg Sweden fredrikw@chalmers.se.

|May 13, 2022

Summary

This summary is machine-generated.

A new smartphone fluorescence microscope enables rapid bacterial identification using peptide nucleic acid (PNA) probes. This cost-effective, portable device offers sensitivity comparable to lab microscopes, ideal for resource-limited settings.

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Last Updated: Sep 23, 2025

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Area of Science:

Biomedical Engineering
Microscopy
Molecular Diagnostics

Background:

Conventional fluorescence microscopy diagnostics are lab-dependent and not suitable for remote or resource-limited settings.
There is a need for portable, cost-effective diagnostic tools for rapid pathogen detection.

Purpose of the Study:

To develop a compact, smartphone-based fluorescence microscope for detecting fluorescently labeled bacteria.
To optimize a peptide nucleic acid (PNA) based fluorescence in situ hybridization (FISH) assay for rapid bacterial identification.

Main Methods:

Development of a smartphone-based fluorescence microscope.
Optimization of a PNA-FISH assay using general nucleic acid stains and species-specific PNA probes.
Evaluation of bacterial detection sensitivity compared to conventional fluorescence microscopy.

Main Results:

The smartphone microscope successfully detected fluorescently labeled bacteria.
Sensitivity was comparable to conventional fluorescence microscopy.
Pathogenic bacteria in powdered infant formula were detected at concentrations as low as 10 CFU per 30 g using PNA-FISH.

Conclusions:

The smartphone-based fluorescence microscope combined with PNA-FISH offers a sensitive and rapid method for bacterial identification.
The platform is cost-effective, portable, and simple to use, making it suitable for resource-limited settings and point-of-care diagnostics.
The assay can be adapted for clinical samples, expanding its diagnostic applications.