A study using LC-MS/MS-based metabolomics to investigate the effects of iron oxide nanoparticles on rat liver

Iron oxide nanoparticles (IONPs) are widely used in food additives, but their metabolic mechanism in the body is still unclear. In this study, male Sprague-Dawley rats were orally administered with IONPs for 28 days to investigate the adverse effect and metabolic mechanism on liver by the combination of traditional toxicology technology and liquid chromatography tandem-mass spectrometry (LC-MS/MS)-based metabolomics. The results showed that IONPs could increase the concentration of blood glucose and the metabolites in the liver of the control and IONPs-treated group were significantly changed. A total of 32 different metabolites were found, including choline, Phosphatidylcholine (PC), Phosphatidylethanolamine (PE), Phosphatidylserine (PS), etc. Pathway analysis based on KEGG database demonstrated that the glycerophospholipid metabolism pathway would be affected. And the expression of the key enzymes of altered metabolomics pathway was further verified at the transcription level. In short, our study clarified oral exposure to IONPs would induce lipid metabolism disorders in the liver of rats, which provided useful information about their safety and potential risks.