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Updated: Sep 23, 2025
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
Published on: July 6, 2014
Mucus secretions from a conditionally reprogrammed primary endocervical cell culture
Daye Park1, Ashok P Reddy2, Phillip A Wilmarth2
1Augusta University/University of Georgia Medical Partnership, Athens, Georgia.
Objective:
To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.
Design:
Experimental.
Setting:
University research center.
Animal(S):
Female rhesus macaque (n = 2).
Intervention(S):
None.
Main Outcome Measure(S):
Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.
Result(S):
We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix.
Conclusion(S):
Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.
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