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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Related Experiment Video

Updated: Sep 23, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

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Single-cell mass spectrometry.

Mohammad Tajik1, Mahroo Baharfar2, William A Donald1

  • 1School of Chemistry, University of New South Wales, Sydney, New South Wales, 2052, Australia.

Trends in Biotechnology
|May 13, 2022
PubMed
Summary
This summary is machine-generated.

Recent advances in mass spectrometry (MS) enable molecular profiling of single cells. This review covers single-cell MS methods for understanding cell heterogeneity and function in biomedicine.

Keywords:
cellular heterogeneitylipidomicsmass cytometrymass spectrometrymetabolomicsproteomicssingle-cell analysis

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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity and function.
  • Recent advances in mass spectrometry (MS) allow for the measurement of numerous biomolecules within individual cells.

Purpose of the Study:

  • To review current single-cell mass spectrometry (MS) methodologies.
  • To discuss the advantages, disadvantages, challenges, and opportunities for single-cell MS.

Main Methods:

  • Review of high-throughput, targeted, mass cytometry-based, and antibody-free single-cell MS approaches.
  • Discussion of proteome and metabolome profiling of single cells.

Main Results:

  • Single-cell MS enables the characterization of molecular heterogeneity.
  • Various MS techniques offer different capabilities for single-cell analysis.

Conclusions:

  • Single-cell MS is a powerful tool for defining cell subtypes and functions.
  • Applications include revealing tumor heterogeneity and high-content drug screening in biomedicine.