Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

175
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
175
Methods of Classification and Identification01:28

Methods of Classification and Identification

267
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
267
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

139
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
139

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing a Core Outcome Measurement Set for Adult ICU Patients, the CoreMS-ICU-A Protocol.

Acta anaesthesiologica Scandinavica·2026
Same author

Conservative Oxygen for Unresponsive Patients after Cardiac Arrest.

The New England journal of medicine·2026
Same author

Evaluating Environmental Ward Performance: Insights From Acoustic, Lighting, and Temperature Monitoring in a Palliative Care Unit.

HERD·2026
Same author

How infection affects the relationship between leukocyte count and mortality risk in intensive care.

Infection·2026
Same author

Evaluation of the high-throughput RespiV <i>PlexP</i><i>lus</i> multiplex assay compared to BioFire Respiratory Panel 2.1 <i>plus</i>.

Microbiology spectrum·2026
Same author

Ivermectin for Critically and Noncritically Ill Hospitalized Patients With COVID-19: Randomized, Embedded, Multifactorial Adaptive Platform Trial for Community-Acquired Pneumonia (REMAP-CAP).

Critical care medicine·2026
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Sep 23, 2025

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

25.6K

Molecular Methodologies for Improved Polymicrobial Sepsis Diagnosis.

Mariam Doualeh1,2,3, Matthew Payne3,4, Edward Litton5,6

  • 1Centre for Molecular Medicine & Innovative Therapeutics, Murdoch University, Murdoch, WA 6150, Australia.

International Journal of Molecular Sciences
|May 14, 2022
PubMed
Summary
This summary is machine-generated.

Polymicrobial sepsis, involving multiple pathogens, leads to poorer patient outcomes. Advanced molecular methods show promise for detecting these complex infections, but require further refinement for clinical use.

Keywords:
PCRmolecular diagnosticspathogen interactionspolymicrobial sepsis

More Related Videos

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
11:25

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

35.9K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

2.0K

Related Experiment Videos

Last Updated: Sep 23, 2025

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

25.6K
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
11:25

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

35.9K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

2.0K

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Polymicrobial sepsis is linked to worse patient outcomes compared to monomicrobial sepsis.
  • Current culture-dependent diagnostic methods often fail to identify all causative pathogens, limiting effective treatment.
  • Understanding pathogen-pathogen interactions is crucial for comprehending polymicrobial sepsis.

Purpose of the Study:

  • To review the clinical significance of polymicrobial sepsis.
  • To discuss improvements needed for molecular microbiology methods in detecting polymicrobial infections.
  • To explore the role of shotgun metagenomics in identifying pathogen interactions in sepsis.

Main Methods:

  • Review of existing literature on polymicrobial sepsis diagnostics.
  • Analysis of challenges and advancements in culture-independent molecular techniques.
  • Discussion of shotgun metagenomics for pathogen interaction analysis.

Main Results:

  • Polymicrobial sepsis presents significant diagnostic challenges with current methods.
  • Molecular methods offer potential but face issues like contamination and host DNA interference.
  • Shotgun metagenomics can reveal complex pathogen interactions in polymicrobial infections.

Conclusions:

  • Improving molecular diagnostic sensitivity is critical for managing polymicrobial sepsis.
  • Shotgun metagenomics holds promise for elucidating pathogen dynamics in polymicrobial sepsis.
  • Further research is needed to optimize molecular techniques for routine clinical application.