Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

353
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
353

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monitoring Nanoparticle Interaction with Murine Breast Cancer Cells Using Multimodal Fluorescence Lifetime Microscopy.

International journal of molecular sciences·2026
Same author

Laser Application in Life Sciences.

International journal of molecular sciences·2023
Same author

Impact of Doxorubicin on Cell-Substrate Topology.

International journal of molecular sciences·2022
Same author

Fluorescence Microscopy-Based Quantitation of GLUT4 Translocation: High Throughput or High Content?

International journal of molecular sciences·2020
Same author

Super-Resolution Live Cell Microscopy of Membrane-Proximal Fluorophores.

International journal of molecular sciences·2020
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Sep 23, 2025

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy
10:18

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Published on: February 3, 2017

9.5K

Lasers in Live Cell Microscopy.

Herbert Schneckenburger1

  • 1Institute of Applied Research, Aalen University, 73430 Aalen, Germany.

International Journal of Molecular Sciences
|May 14, 2022
PubMed
Summary
This summary is machine-generated.

Lasers are vital for advanced live cell microscopy techniques, including 3D and super-resolution imaging. Their unique properties enable applications like fluorescence diagnosis, photodynamic therapy, and laser-assisted optoporation in cell biology.

Keywords:
FLIMFRETTIRFMconfocal microscopylaser micromanipulationlight sheet microscopyoptical tweezersphotodynamic therapyspectral imagingsuper-resolution

More Related Videos

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
07:40

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

1.4K
Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

6.7K

Related Experiment Videos

Last Updated: Sep 23, 2025

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy
10:18

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Published on: February 3, 2017

9.5K
A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
07:40

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

1.4K
Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

6.7K

Area of Science:

  • Biomedical Optics
  • Cell Biology
  • Microscopy

Background:

  • Lasers offer unique properties such as coherent radiation and diffraction-limited focusing, making them increasingly important in live cell microscopy.
  • These properties are essential for various advanced imaging techniques, including 3D microscopy (confocal, light sheet, TIRF) and super-resolution microscopy.
  • Lasers are also crucial for specialized techniques like spectral imaging, fluorescence lifetime imaging (FLIM), and optical manipulation (optical tweezers).

Purpose of the Study:

  • To highlight the expanding role and diverse applications of lasers in live cell biology.
  • To showcase exemplary laser applications in live cell research.
  • To demonstrate the broad potential of laser technology in cell biology and biomedicine.

Main Methods:

  • Review and discussion of laser properties relevant to microscopy.
  • Description of laser integration in 3D microscopy techniques (confocal, light sheet, TIRF).
  • Explanation of laser use in super-resolution, spectral imaging, FLIM, and optical manipulation.
  • Outline of three exemplary laser applications: fluorescence diagnosis (with FRET), photodynamic therapy, and laser-assisted optoporation.

Main Results:

  • Lasers are indispensable for achieving high resolution and advanced imaging in live cell microscopy.
  • Specific laser properties facilitate techniques like fluorescence diagnosis, FRET, photodynamic therapy, and optoporation.
  • Laser microbeams are effective tools for optical tweezers and cell micromanipulation.

Conclusions:

  • Lasers significantly enhance capabilities in live cell imaging and manipulation.
  • The unique properties of lasers support a wide range of cutting-edge applications in cell biology.
  • Lasers represent a powerful and versatile tool with broad implications for biomedical research.