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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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When Super-Resolution Localization Microscopy Meets Carbon Nanotubes.

Somen Nandi1,2, Karen Caicedo1,2, Laurent Cognet1,2

  • 1Laboratoire Photonique Numérique et Nanosciences, Université de Bordeaux, UMR 5298, 33400 Talence, France.

Nanomaterials (Basel, Switzerland)
|May 14, 2022
PubMed
Summary
This summary is machine-generated.

Super-resolution microscopy (SRM) revolutionizes biological imaging by achieving nanoscale resolution. This review explores SRM applications using nanomaterials, particularly single-walled carbon nanotubes (SWCNTs), for advanced bio-imaging.

Keywords:
bionanotechnologynanosciencenanoscopynear-infrared (NIR) probessingle-molecule studysingle-particle tracking (SPT)single-walled carbon nanotubes (SWCNTs)super-resolution microscopy (SRM)

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Area of Science:

  • Optics and Photonics
  • Nanotechnology
  • Biophysics

Background:

  • Super-resolution microscopy (SRM) overcomes the diffraction limit, enabling nanometer-scale imaging in biological specimens.
  • SRM bridges the gap between cellular and molecular structural knowledge, previously limited to electron microscopy.
  • Advances in controlling fluorescent molecules and developing novel nanostructures are key to SRM progress.

Purpose of the Study:

  • To review recent developments in super-resolution imaging utilizing nanomaterials.
  • To highlight the application of single-walled carbon nanotubes (SWCNTs) as fluorescent nanomaterials in SRM.
  • To demonstrate the integration of SRM with SWCNTs for nanoscale imaging applications.

Main Methods:

  • Focus on super-resolution imaging techniques.
  • Utilize fluorescent nanomaterials, specifically single-walled carbon nanotubes (SWCNTs).
  • Apply SRM strategies for imaging "in", "of", and "with" SWCNTs.

Main Results:

  • SWCNTs serve as effective photoluminescent emitters in the near-infrared (NIR) spectrum.
  • SRM techniques enable nanoscale imaging of SWCNTs and their integration into biological systems.
  • Demonstrated potential for fundamental nanomaterial science and biological imaging applications.

Conclusions:

  • Nanomaterials, especially SWCNTs, offer new avenues for super-resolution microscopy.
  • SRM combined with SWCNTs facilitates detailed nanoscale visualization in biological contexts.
  • This synergy advances both nanomaterial applications and our understanding of biological structures.