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Related Experiment Video

Updated: Sep 23, 2025

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Polymeric Electrochemical Sensor for Calcium Based on DNA.

Mohsen M Zareh1, Soha F Mohamed1, Anas M Elsheikh1

  • 1Department of Chemistry, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.

Polymers
|May 14, 2022
PubMed
Summary

This study developed a novel calcium (Ca2+) selective sensor using deoxyribonucleic acid (DNA) in plastic membranes. The sensor demonstrated excellent performance and selectivity for calcium detection in various real-world samples.

Keywords:
calcium determinationcalcium sensordeoxyribonucleic acid ionophore

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Plastic membranes are crucial in sensor technology.
  • Deoxyribonucleic acid (DNA) shows potential as an electroactive material for ion sensing.
  • Developing selective and efficient calcium sensors is important for various applications.

Purpose of the Study:

  • To develop and characterize a novel calcium (Ca2+) selective sensor utilizing DNA as the electroactive material.
  • To optimize the membrane composition for enhanced sensor performance.
  • To evaluate the sensor's selectivity, response time, and applicability in real samples.

Main Methods:

  • Fabrication of polyvinyl chloride (PVC) based plastic membranes incorporating DNA as an ionophore.
  • Testing different plasticizers (DEP, DOP, NPOE) and optimizing the membrane composition (DNA: DOP: PVC).
  • Electrochemical characterization including calibration, pH range determination, response time, and selectivity studies.

Main Results:

  • A sensor with a specific composition (120 mg PVC, 60 mg DOP, 2 mg DNA) exhibited optimal performance.
  • The sensor achieved a slope of 30 mV decade-1 within an optimal pH range of 5.7-9.5.
  • Fast response time (2-3 s), long working period (3 weeks), and excellent Ca2+ selectivity were observed.

Conclusions:

  • Plastic membranes incorporating DNA can function as effective Ca2+ selective sensors.
  • The developed sensor shows high selectivity and rapid response, making it suitable for practical applications.
  • The sensor was successfully applied for calcium determination in real samples like fruits, milk, and dairy products.