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Decrease in triiodothyronine binding sites in chick embryo erythrocytes during early development.

A K Dasmahapatra, C R Thomas, E Frieden

    Developmental Biology
    |April 1, 1987
    PubMed
    Summary
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    Thyroid hormone (TH) receptors are found in chick embryo erythrocyte nuclei. These binding sites significantly decrease during development, suggesting a regulatory role in embryogenesis.

    Area of Science:

    • Endocrinology
    • Developmental Biology
    • Cell Biology

    Background:

    • Specific thyroid hormone (TH) binding sites have been identified in the nuclei of developing chick embryo erythrocytes.
    • These binding characteristics align with those of established TH receptors.

    Purpose of the Study:

    • To characterize the binding properties of TH receptors in chick erythrocyte nuclei.
    • To investigate the changes in the number and affinity of these receptors during chick embryogenesis.

    Main Methods:

    • In vitro nuclear triiodothyronine (T3) saturation analysis using [125I]T3 on isolated erythrocyte nuclei.
    • Scatchard analysis to determine binding site characteristics and affinity.
    • Serial studies across different days of chick embryogenesis to track receptor levels.

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    Main Results:

    • Erythrocyte nuclei possess a single class of reversible TH binding sites with temperature-dependent dissociation.
    • Triiodothyronine (T3) exhibits higher affinity than thyroxine (T4), with Triac showing the highest affinity among tested analogs.
    • The number of T3 binding sites per nucleus dramatically decreases from Day 5 (maximal) to Day 20 of embryogenesis, with minimal sites in adult and baby chickens.
    • Binding affinity (Kd) remained constant around 20 pM at 37°C throughout the study period.

    Conclusions:

    • Chick erythrocyte nuclei contain functional TH receptors with specific binding affinities.
    • A significant decline in erythrocyte nuclear T3 receptor number occurs during embryogenesis, potentially linked to increasing plasma TH levels or changes in erythrocyte populations.