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Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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N6-Methyladenosine dynamic changes and differential methylation in wheat grain development.

Wenxiang Li1, Yi Yu1, Xuanrong Chen1

  • 1College of Agronomy, Anhui Agricultural University, Hefei, 230036, Anhui, China.

Planta
|May 14, 2022
PubMed
Summary

N6-methyladenosine (m6A) modification dynamics in wheat seed development reveal more changes in later stages. Protein and starch synthesis pathways are enriched during late wheat grain development, impacting yield and quality.

Keywords:
Differential methylationGenomeGrain developmentRegulationTriticum aestivumm6A methylation

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Area of Science:

  • Plant Biology
  • Epigenetics
  • Molecular Biology

Background:

  • Wheat seed development is crucial for yield and quality, influenced by genetics, epigenetics, and environment.
  • N6-methyladenosine (m6A) modification is a dynamic epigenetic process regulating plant development and stress responses.
  • Understanding m6A's role in wheat grain development is essential for improving crop traits.

Purpose of the Study:

  • To characterize the dynamic changes of m6A modification during wheat seed development.
  • To identify genes and pathways regulated by m6A during different stages of grain development.
  • To elucidate the role of m6A in wheat yield and quality determination.

Main Methods:

  • Wheat grain samples were collected at 10, 20, and 30 days post-anthesis (DPA).
  • m6A-sequencing (m6A-seq) was employed to identify m6A peaks and methylated genes.
  • KEGG enrichment analysis was performed to identify significantly enriched pathways.

Main Results:

  • m6A modification peaks were identified across wheat seed development stages, primarily enriched in 3'UTRs.
  • A significant number of genes showed dynamic m6A methylation patterns, with more genes affected in late development.
  • Protein and starch synthesis pathways were notably enriched in the later stages of wheat seed development.

Conclusions:

  • m6A modification plays a dynamic regulatory role throughout wheat seed development, with increased activity in later stages.
  • Enrichment of protein and starch synthesis pathways in late development suggests m6A's involvement in grain filling and quality.
  • These findings provide novel insights into the epigenetic regulation of wheat grain development and potential targets for crop improvement.