Related Experiment Video
Updated: Sep 23, 2025
07:20
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
Published on: June 14, 2018
12.6K
Gene knockout in cellular immunotherapy: Application and limitations
Xiaomei Zhang1, Xin Jin2, Rui Sun1
1School of Medicine, Nankai University, Tianjin, China.
Cancer Letters
|May 15, 2022
Summary
Gene editing technologies like CRISPR/Cas9 are advancing cellular immunotherapy, including CAR-T cell therapy. Ongoing research focuses on improving safety and efficacy through advanced gene editing and delivery methods for better therapeutic outcomes.
Area of Science:
- Biotechnology
- Immunotherapy
- Genetics
Background:
- Cellular immunotherapy, including CAR-T cell therapy, shows promise but requires modifications for enhanced safety and efficacy.
- Gene editing technologies have evolved significantly, offering precise tools for cellular modification.
Purpose of the Study:
- To summarize current clinical trials and fundamental research in genome editing therapy.
- To review in vivo gene delivery systems and identify limitations for improved gene therapy strategies.
Main Methods:
- Review of ongoing clinical trials and fundamental research in genome editing.
- Analysis of existing in vivo gene delivery systems and their associated challenges.
Main Results:
- Significant advancements in gene editing technologies, from RNA interference (RNAi) to CRISPR/Cas9.
- Identification of limitations in current in vivo delivery systems for gene therapy applications.
Conclusions:
- Genome editing holds substantial potential for advancing cellular immunotherapy.
- Further development of efficient and safe in vivo delivery methods is crucial for successful gene therapy implementation.
Related Concept Videos
01:16In-vitro Mutagenesis
14.4K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.4K
01:27Tumor Immunotherapy
681
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
681
01:27Cytotoxic T Cells-mediated Immune Response
2.7K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
2.7K