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A novel function of collagen/PCL nanofiber interaction with MSCs in osteoarthritis is potentiation its
Gehan Abd-Elfatah Tawfeek1, Heba Ahmed Esaily2
1Department of Clinical pathology, Faculty of Medicine, Menofia University, Egypt.
Background:
The previous studies investigated the scaffold as mechanical carrier to stem cells or growth factors as transplantation therapy. This study aimed to investigate the effect of nanofiber scaffold on immunomodulatory properties of MSCs in osteoarthritis (OA) and the mechanism of this effect.
Methods:
Collagen coated PCL nanofiber was prepared and examined by SEM. Mononuclear cells (MNCs) from the peripheral blood (P.B) and synovial fluid (SF) cultured with BMMSCs after stimulation by PHA using two types of co-culture (MSCs/Nano- and MSCs/nano+). Using flowcytometry, CD4, CD8, CD28, activation markers CD38 and CD23, regulatory CD4CD25high foxp3, CD14, IL17 by ELISA and cell proliferation using CCK-8 were assessed. ICAM mRNA was assessed by qRT-PCR and flowcytometry.
Results:
MSCs/nano+ showed more significant inhibitory effect of MSCs regarding CD4, CD8, CD28, CD28 MFI, CD38, CD38 MFI, CD23, CD23 MFI, CD14, IL17 (p < 0.001) and higher level of Treg cells (p < 0.001) than nano- with difference regarding cell proliferation (p = 0.275). Also, the immunomodulatory effect of MSCs culture on nano+ on lymphocyte separated from S·F of osteoarthritis patients more powerful than nano-. The secretory function of MSCs culture on nano+ is more beneficial than that of nano-. Nano+ showed higher level of ICAM mRNA and CD54 than nano-.
Conclusion:
The present study proved a novel function of nanofiber by increasing the immunomodulatory effect of the MSCs in osteoarthritis through increased the ICAM expression and this promising for increase the success of MSCs transplantation in OA.
