Biosynthesis of (R)-(+)-perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase
Chao Sun1,2, Rubing Zhang2, Congxia Xie1
1State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao P. R. China.
Abstract:
(R)-(+)-perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)-(+)-perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)-(+)-perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)-supplied pathway resulted in a 4-fold improvement of (R)-(+)-perillyl alcohol titer. Subsequently, combined engineering of p-cymene monooxygenase (CymA) expression and module for NADPH regeneration exhibited a 15.4-fold increase of titer over the initial strain S02. Finally, 453 mg/L (R)-(+)-perillyl alcohol was achieved in fed-batch fermentation, which is the highest (R)-(+)-perillyl alcohol titer in E. coli.
Related Concept Videos
Biosynthesis of Lipids
Biosynthesis in Bacteria
Biosynthesis of Nucleic Acids
Amino Acid Biosynthetic Pathways
Other Glycolytic Pathways
Stringent Response in E. coli


