Related Experiment Video

Updated: Sep 23, 2025

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

15.4K

Fluorescent Indicators For Biological Imaging of Monatomic Ions

Sheng-Yi Wu1, Yi Shen1, Irene Shkolnikov1,2

  • 1Department of Chemistry, University of Alberta, Edmonton, AB, Canada.

Frontiers in Cell and Developmental Biology
|May 16, 2022
PubMed

Keywords:
fluorescence imaginggenetically encoded indicatorsmonatomic ionsprotein engineeringsmall molecule-based indicators

More Related Videos

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.1K
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

14.2K

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
510
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.5K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
790