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Updated: Sep 23, 2025

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
Published on: November 29, 2013
Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision
1National Institute of Standards and Technology, Bioprocess Measurement Group, Biomolecular Measurement Division, Gaithersburg, MD 20899, USA.
Abstract:
This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to ±0.05 oC are also included in this tutorial.

