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African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified

Gallardo Carmina1, R Nieto2, M Arias2

  • 1European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal, CISA-INIA/CSIC, Valdeolmos, Madrid, Spain. gallardo@inia.es.

Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2022
PubMed

Keywords:
ASFV antibodiesAfrican swine fever virus (ASFV)ELISASoluble cytoplasmic antigen

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.