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Updated: Sep 23, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Published on: June 17, 2012
The WKRY transcription factor MdWRKY75 regulates anthocyanins accumulation in apples (Malus domestica)
Mengyu Su1, Weifang Zuo1, Yicheng Wang1
1State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, China; and Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production in Shandong, Tai-An, China.
Abstract:
Anthocyanins play important roles in plant secondary metabolism. Although previous studies have identified many transcription factors (TFs) that participate in the synthetic pathway of anthocyanins, the regulation mechanism of the pathway remain poorly understood. In this study, we identified a WRKY Group IIc TF, MdWRKY75, which contained a typical WRKYGQK heptapeptide sequence and a C2 H2 -zinc finger structure. Subcellular localisation assays found that MdWRKY75 was located in the nucleus. Overexpression of MdWRKY75 promoted the accumulation of anthocyanins in apple (Malus domestica L.) 'Orin' calli. MdWRKY75 mainly stimulated the accumulation of anthocyanins by binding to the promoter of MYB transcription factor, MdMYB1 . Our research could provide new insights into how WRKY TFs regulate the accumulation of anthocyanins in apples.
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