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Novel electron microscopic staining method using traditional dye, hematoxylin.

Hiroyuki Sasaki1, Hisako Arai2, Emi Kikuchi2

  • 1Department of Occupational Therapy, School of Rehabilitation, Tokyo Professional University of Health Sciences, Tokyo, Japan. h-sasaki@tpu.ac.jp.

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|May 16, 2022
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Summary
This summary is machine-generated.

Researchers found a new, safe staining method for electron microscopy using Mayer's hematoxylin and Reynold's lead citrate. This alternative avoids radioactive uranyl acetate, offering comparable results for ultrathin sections.

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Area of Science:

  • Biological electron microscopy
  • Microscopy staining techniques

Background:

  • Uranyl acetate (UA) is a standard stain for ultrathin sections in electron microscopy.
  • UA is radioactive and subject to strict international regulations, necessitating safer alternatives.

Purpose of the Study:

  • To develop an easy-to-use and safe alternative staining method for ultrathin sections.
  • To identify commercial light microscopic dyes suitable for electron microscopy.

Main Methods:

  • Evaluation of various commercial light microscopic dyes.
  • Comparison of staining results using Mayer's hematoxylin and Reynold's lead citrate against UA and Reynold's lead citrate.

Main Results:

  • Mayer's hematoxylin followed by Reynold's lead citrate demonstrated comparable staining quality to UA and Reynold's lead citrate.
  • The new method proved effective for ultrathin section staining.

Conclusions:

  • Mayer's hematoxylin and Reynold's lead citrate offer a reliable and promising alternative to UA staining.
  • This method addresses safety concerns associated with radioactive staining materials.