Controlled Alternate Layer-by-Layer Assembly of Lectins and Glycopolymers Using QCM-D

Layer-by-layer (LBL) assembly of concanavalin A (Con A), peanut agglutinin (PNA) plant lectins, and well-defined synthetic glycopolymers via their biological affinities have been prepared using a quartz crystal microbalance with dissipation monitoring (QCM-D). We demonstrate the use of mannose/galactose glycopolymers as lectin binders due to their selective binding to Con A/PNA, respectively. A detailed analysis of the adsorption processes and the adsorbed layer are provided and tuning the composition of multilayers using a series of well-defined glycopolymers differing only in the pendant sugar ratio is discussed.