Related Experiment Video
Updated: Sep 23, 2025

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Published on: February 3, 2023
Immobilization and Photocurrent Activity of a Light-Harvesting Antenna Complex II, LHCII, Isolated from a Plant on
Morio Nagata1, Mizuki Amano1, Takashi Joke1
1Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.
Abstract:
A light-harvesting (LH) antenna complex II, LHCII, isolated from spinach was immobilized onto an indium tin oxide (ITO) electrode with dot patterning of 3-aminopropyltriethoxysilane (APS) by utilizing electrostatic interactions between the cationic surface of the electrode and the anionic surface of stromal side of the LHCII polypeptide. Interestingly, the illumination of LHCII assembled onto the ITO electrode produced a photocurrent response that depends on the wavelength of the excitation light. Further, LHCII was immobilized onto a TiO2 nanostructured film to extend for the development of a dye-sensitized biosolar cell system. The photocurrent measured in the iodide/tri-iodide redox system of an ionic liquid based electrolyte on the TiO2 system showed remarkable enhancement of the conversion efficiency, as compared to that on the ITO electrode.
More Related Videos
Related Concept Videos
The Antenna Complex
Photosystem II
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
Photosystems
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Photoreceptors and Plant Responses to Light
Photosystem I
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...

