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Biocompatible Films of Collagen-Procyanidin for Wound Healing Applications
K Sivaraman1, P Sujitha1, A Arunkumar1
1Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Tamil Nadu, Vellore, 632014, India.
Applied Biochemistry and Biotechnology
|May 17, 2022
Summary
Cassia auriculata leaf polyphenols, identified as procyanidin B, enhance collagen film stability and accelerate wound healing. These findings suggest potential for improved collagen-based wound dressings.
Area of Science:
- Biomaterials Science
- Pharmacognosy
- Wound Healing Research
Background:
- Collagen films are promising for wound healing but require enhanced stability and efficacy.
- Cassia auriculata (CA) leaves contain polyphenols with potential therapeutic properties.
Purpose of the Study:
- To investigate the wound healing potential of Cassia auriculata leaf extract (EEAF) incorporated into collagen films.
- To evaluate the effect of EEAF on collagen film stability and physical properties.
Main Methods:
- Phytochemical analysis and purification of CA extract to identify active compounds.
- Characterization of the active compound using HP-TLC, FTIR, ESI-FT-MS/MS, and 1H NMR.
- In vitro scratch assay on fibroblast cell lines and in vivo wound healing studies in rats.
Main Results:
- Procyanidin B was identified as the active polyphenol in EEAF.
- EEAF-treated collagen films demonstrated enhanced physical strength and improved in vitro and in vivo wound closure.
- Bovine tendon collagen films with EEAF showed superior performance compared to fish collagen films.
Conclusions:
- Cassia auriculata leaf extract, specifically procyanidin B, significantly enhances collagen film properties and accelerates wound healing.
- EEAF-incorporated collagen films represent a promising advancement for wound dressing applications.
