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A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of plum viroid I
Rachelle Bester1, Hans J Maree1
1Citrus Research International, PO Box 2201, Matieland 7602, South Africa; Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Abstract:
Plum viroid I (PlVd-I) is found in marbling and corky flesh diseased plum trees in South Africa. In this study a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the high-throughput detection of PlVd-I was developed. This assay can be performed on crude extracts and detection can either be a pH dependent colorimetric reaction or a real-time fluorescent signal reaction. The false discovery rate was shown to be low and no decrease in sensitivity was detected compared to RT-PCR. The RT-LAMP assay allows for the fast and cost-effective detection of PlVd-I that will curtail the distribution of infected plant material.
