Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular characterisation of a novel citrus-infecting emaravirus, citrus emaravirus 1.

Archives of virology·2025

Same author

Grapevine leafroll-associated virus 3: a global threat to grapevine and wine industries but a gold mine for scientific discovery.

Journal of experimental botany·2025

Same author

Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation.

Plant disease·2025

Same author

Applying infectious clones and untargeted metabolite profiling to characterize citrus tristeza virus-induced stem pitting in citrus.

Scientific reports·2024

Same author

Investigating Protein-Protein Interactions Between Grapevine Leafroll-Associated Virus 3 and <i>Vitis vinifera</i>.

Phytopathology·2023

Same author

Concerning the Etiology of Syrah Decline: A Fresh Perspective on an Old and Complex Issue Facing the Global Grape and Wine Industry.

Viruses·2023

Search research articles

Related Experiment Video

Updated: May 23, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Complete genome sequence of fig leaf mottle-associated virus 2.

Rachelle Bester^1,2, Shannon Goodchild¹, Hans J Maree^3,4

¹Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.

Archives of Virology

|March 12, 2025

Summary

The complete genome of fig leaf mottle-associated virus 2 (FLMaV2) was sequenced, revealing unique characteristics. Phylogenetic analysis suggests FLMaV2, LChV2, and YaV1 may form a new genus within the Closteroviridae family.

More Related Videos

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Related Experiment Videos

Last Updated: May 23, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Area of Science:

Plant virology
Molecular biology
Phylogenetics

Background:

Fig leaf mottle-associated virus 2 (FLMaV2) is a positive-sense single-stranded RNA virus.
Its genome organization is similar to little cherry virus 2 (LChV2) and yam asymptomatic virus 1 (YaV1).
These viruses are currently classified under the genus Ampelovirus but exhibit atypical features.

Purpose of the Study:

To determine the complete genome sequence of FLMaV2.
To analyze the phylogenetic relationships of FLMaV2 with other viruses in the family Closteroviridae.
To investigate the potential taxonomic classification of FLMaV2, LChV2, and YaV1.

Main Methods:

Reverse transcription-polymerase chain reaction (RT-PCR).
Rapid amplification of cDNA ends (RACE).
Phylogenetic analysis using amino acid sequences of RNA-dependent RNA polymerase and coat protein.

Main Results:

The complete genome sequence of FLMaV2 was determined to be 16,925 nucleotides.
FLMaV2 shares genome organization similarities with LChV2 and YaV1.
Phylogenetic analysis placed FLMaV2, LChV2, and YaV1 together, distinct from typical ampeloviruses.

Conclusions:

FLMaV2 possesses an unusual genome organization within the Ampelovirus genus.
FLMaV2, LChV2, and YaV1 exhibit distinct phylogenetic characteristics.
These findings suggest the potential establishment of a new genus for FLMaV2, LChV2, and YaV1 within the Closteroviridae family.