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Identification of Interactions between Proteins Encoded by Grapevine Leafroll-Associated Virus 3
Ilani Mostert1, Rachelle Bester1,2, Johan T Burger1
1Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Viruses
|January 21, 2023
Summary
This study investigated protein interactions in grapevine leafroll-associated virus 3 (GLRaV-3), revealing self-interactions and novel protein partnerships. These findings offer insights into ampelovirus virion assembly and host-pathogen interactions.
Area of Science:
- Plant Virology
- Molecular Biology
- Protein-Protein Interactions
Background:
- Proteins of the genus *Ampelovirus* (family *Closteroviridae*) roles are often inferred by sequence homology.
- Grapevine leafroll-associated virus 3 (GLRaV-3) is a significant pathogen impacting grapevine health.
Purpose of the Study:
- To investigate protein-protein interactions within GLRaV-3 using experimental methods.
- To elucidate the roles of specific GLRaV-3 proteins, including p5, HSP70 homolog, coat protein, and p20B, in viral processes.
Main Methods:
- Yeast two-hybrid assays were employed to detect protein interactions.
- Bimolecular fluorescence complementation (BiFC) assays were utilized to confirm interactions in a cellular context.
Main Results:
- The p5 movement protein, HSP70 homolog (HSP70h), coat protein, and p20B of GLRaV-3 were found to self-interact.
- HSP70h interacts with the coat protein, and p20B interacts with HSP70h, the major coat protein, and the minor coat protein.
- The p5 protein's interaction mechanism remains unclear due to a missing cysteine residue.
Conclusions:
- GLRaV-3 virion assembly shares similarities but also differs from other *Closteroviridae* genera.
- Interactions involving p20B suggest its role in host silencing suppression and potentially other viral replication stages.
Keywords:
AmpelovirusClosteroviridaebimolecular fluorescence complementationprotein-protein interactionsvirion assemblyyeast two-hybridMore Related Videos
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