Related Experiment Video
Updated: Sep 22, 2025

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Published on: April 28, 2021
DNA direct reversal repair and alkylating agent drug resistance
Roberto Gutierrez1,2, Timothy R O'Connor1
1Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA.
Abstract:
DNA direct reversal repair (DRR) is unique in that no DNA synthesis is required to correct the error and therefore repair via such mechanisms are error-free. In humans, DRR is carried out by two different pathways: the O6-methylguanine-DNA methyltransferase (MGMT) and the alkylated DNA repair protein B (AlkB) homologs. The use of alkylating agents is the standard of care for many cancers. However, the use of those drugs is usually halted when resistance develops. This review will examine repair of alkylating agent damage mediated by DRR, resistance mechanisms and potential ways to overcome such resistance.
Related Concept Videos
Overview of DNA Repair
Chemically...
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Base Excision Repair
The first step of...
Treatment Resistant Cancers
Homologous Recombination
Fixing Double-strand Breaks

