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Updated: Sep 22, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Sensitive Plant N-Terminome Profiling with HUNTER.

Fatih Demir^1,2, Andreas Perrar^1,3, Melissa Mantz^1,3

¹Central Institute for Engineering, Electronics and Analytics, Forschungszentrum Jülich, Jülich, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|May 18, 2022

Summary

This summary is machine-generated.

Protein N-termini reveal crucial proteoform information but are hard to detect. The High-efficiency Undecanal-based N-Termini EnRichment (HUNTER) method efficiently enriches these peptides from small samples for plant N-terminome profiling.

Keywords:

HUNTER N-terminal protein modifications N-termini N-terminomics Positional proteomics Proteolysis Proteomics

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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

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Published on: January 21, 2020

Area of Science:

Proteomics
Biochemistry
Plant Science

Background:

Protein N-termini are vital for identifying distinct proteoforms generated by processing, modification, or alternative translation.
N-terminal peptides are typically low abundance and require enrichment for effective analysis in shotgun proteomics.
Existing enrichment methods often necessitate large sample amounts, limiting their application.

Purpose of the Study:

To present an optimized HUNTER protocol for sensitive plant N-terminome profiling.
To demonstrate the utility of HUNTER for analyzing limited proteome samples.
To detail sample preparation, N-terminal peptide enrichment, and mass spectrometry analysis steps.

Main Methods:

High-efficiency Undecanal-based N-Termini EnRichment (HUNTER) protocol.
Application to limited sample sources (micrograms of proteome).
Mass spectrometry-based N-terminome profiling.

Main Results:

Successful enrichment of N-terminal peptides from small plant proteome samples.
Sensitive profiling of the plant N-terminome.
Demonstration of HUNTER's speed and sensitivity.

Conclusions:

The HUNTER protocol enables sensitive N-terminome profiling from limited plant samples.
HUNTER is a valuable tool for proteoform discovery and modification analysis in plants.
This method expands the possibilities for studying plant proteomes with minimal starting material.