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Updated: Sep 22, 2025
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
[The effect of chronic kidney disease on periodontitis-related microorganisms in mice]
Xiao-Xin Ma1, Sheng-Zhong Duan, Xi-Ping Feng
1College of Stomatology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.
Purpose:
To explore the effect of chronic kidney disease on the composition of oral microbial community in mice and find the significant species.
Methods:
Twenty C57BL/6 male mice were randomly divided into 4 groups: healthy control group (HC), periodontitis group (PD), chronic kidney disease group (CKD) and chronic kidney disease and periodontitis group (CKD+PD). The periodontitis model was created in the fourth week when the chronic kidney disease model was established, and then the mice were sacrificed in the sixth week. Histopathological analysis of the kidney was performed by H-E staining and Masson's trichrome staining. Alveolar bone resorption of maxilla was analyzed by micro-CT analysis. The third-generation full-length sequencing of 16SrRNA gene was used to analyze the composition of oral microbial community among groups. Statistical analysis was performed using SPSS 24.0 software package.
Results:
There were significant differences in alveolar bone resorption, the richness of species and the overall composition of the microbial community among the four groups (P＜0.001). In CKD group, Streptococcus azizii had the most significant abundance. Escherichia coli was the most significantly different species among identifiable bacteria in CKD+PD group, while Staphylococcus lentus and Lactobacillus murinus were species with the most significant differences in HC group and PD group, respectively.
Conclusions:
The composition of the oral microbial community was significantly different among four groups with significant species.
Insights
Chronic kidney disease significantly alters the oral microbial community in mice. Specific bacterial species like Streptococcus azizii and Escherichia coli showed significant changes, impacting oral health.
Area of Science:
- Oral microbiology
- Nephrology
- Periodontology
Background:
- Chronic kidney disease (CKD) is associated with systemic health issues.
- The oral microbiome plays a crucial role in maintaining oral and overall health.
- Understanding the interplay between CKD and oral microbiota is essential for potential therapeutic interventions.
Purpose of the Study:
- To investigate the impact of chronic kidney disease on the oral microbial community composition in a mouse model.
- To identify specific bacterial species that are significantly altered in the presence of CKD.
- To examine the combined effects of CKD and periodontitis on the oral microbiome.
Main Methods:
- A mouse model involving four groups: healthy control (HC), periodontitis (PD), chronic kidney disease (CKD), and combined CKD and periodontitis (CKD+PD).
- Induction of periodontitis and CKD models, followed by sample collection at six weeks.
- Histopathological analysis of kidney and maxilla (for alveolar bone resorption via micro-CT).
- 16S rRNA gene sequencing for oral microbial community analysis.
Main Results:
- Significant differences in alveolar bone resorption, species richness, and overall microbial community composition were observed across all groups (P<0.001).
- In the CKD group, *Streptococcus azizii* exhibited the highest abundance.
- In the CKD+PD group, *Escherichia coli* was the most significantly altered species, while *Staphylococcus lentus* and *Lactobacillus murinus* showed the most significant differences in the HC and PD groups, respectively.
Conclusions:
- Chronic kidney disease significantly alters the oral microbial community structure in mice.
- Specific bacterial species are identified as significantly affected by CKD, alone or in combination with periodontitis.
- These findings highlight the intricate relationship between kidney health and the oral microbiome.
