Development of a highly sensitive glucose nanocomposite biosensor based on recombinant enzyme from corn

Area of Science:

• Biotechnology
• Nanotechnology
• Electrochemistry

Background:

• Enzymes are essential biocatalysts for biomolecule production.
• Plants offer a cost-effective source for recombinant enzyme production.
• Electrochemical enzyme immobilization enhances biosensor performance and stability.

Purpose of the Study:

• To develop a sensitive and selective glucose biosensor.
• To explore the use of plant-derived enzymes in biosensor applications.
• To investigate the synergistic effects of conductive polymers and nanoparticles in biosensor design.

Main Methods:

• Construction of a glucose biosensor using polyaniline (PANI), plant-produced manganese peroxidase (PPMP), gold nanoparticles (AuNPs), and glucose oxidase (GOx).
• Electrochemical characterization using linear sweep voltammetry and cyclic voltammetry.
• Assessment of biosensor selectivity against common interfering substances.

Main Results:

• The PANI-AuNPs-GOx-PPMP/Au electrode demonstrated superior sensing performance.
• Achieved a wide linear range (0.005-16.0 mM) and a low detection limit (0.001 mM) for glucose.
• The biosensor exhibited selectivity in the presence of ascorbic acid, dopamine, aspartame, and caffeine.

Conclusions:

• A nanocomposite biosensor combining plant-derived Mn peroxidase, conductive polymers, and AuNPs shows significant promise for glucose detection.
• This biosensor technology holds potential for economic impact in food industry quality control.
• The developed electrochemical method offers an efficient and stable approach for enzyme immobilization.