Rapid and Sensitive Glucose Detection Using Recombinant Corn Mn Peroxidase and Advanced Voltammetric Methods

Area of Science:

• Electrochemistry
• Biosensors
• Biotechnology

Background:

• Glucose detection is crucial for medical diagnostics and food safety.
• Existing methods often lack sensitivity, selectivity, or portability.
• Development of novel biosensors is needed for improved glucose monitoring.

Purpose of the Study:

• To develop a novel disposable electrochemical biosensor for sensitive and selective glucose detection.
• To integrate recombinant corn-derived manganese peroxidase with other components for enhanced sensor performance.
• To evaluate the sensor's performance using square wave voltammetry (SWV) and linear sweep voltammetry (LSV).

Main Methods:

• Fabrication of gold-modified screen-printed electrodes.
• Integration of recombinant corn-derived manganese peroxidase, glucose oxidase, bovine serum albumin, and gold nanoparticles.
• Electrochemical analysis using square wave voltammetry (SWV) and linear sweep voltammetry (LSV).

Main Results:

• Linear sweep voltammetry (LSV) detected glucose in the range of 0.001-6.5 mM (R² = 0.9913; LOD = 0.50 µM).
• Square wave voltammetry (SWV) detected glucose in the range of 0.0006-6.5 mM (R² = 0.998; LOD = 0.29 µM).
• The biosensor exhibited excellent selectivity against common electroactive species and rapid response times.

Conclusions:

• The developed disposable electrochemical biosensor provides highly sensitive and selective glucose detection.
• The sensor's performance characteristics make it suitable for point-of-care diagnostics and food quality monitoring.
• The integration of specific enzymes and nanomaterials enhances sensor stability and signal transduction.