Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.9K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluid mechanics of thin blood films to detect anemia and sickle cell disease.

Lab on a chip·2026
Same author

Corrigendum to "Evaluation of Mechanical Properties of TiO2-free Tablet Coatings" [Journal of Pharmaceutical Sciences/ Volume 114, Issue 10, 10 2025, 103942].

Journal of pharmaceutical sciences·2025
Same author

Evaluation of mechanical properties of TiO<sub>2</sub>-free tablet coatings.

Journal of pharmaceutical sciences·2025
Same author

Effective modulus of particle-packing containing hard and soft particles.

Soft matter·2025
Same author

A viscosity measurement technique for ultra-low sample volumes.

Soft matter·2024
Same author

Critical cracking thickness of drying polymer films.

Soft matter·2023
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
Same journal

Synergistic mode-field pre-expansion and geometric compression in hetero-structured microfibers for ultrasensitive glucose sensing.

Biosensors & bioelectronics·2026
Same journal

An amplification-free dual-readout biosensor integrating colorimetry and single-particle counting for ultrasensitive miRNA detection in esophageal cancer.

Biosensors & bioelectronics·2026
Same journal

An all-in-one microfluidic system via data-driven design for on-site genotyping of genetically modified foods.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Sep 22, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

23.8K

A low-cost flow cell for flow cytometry.

Mahrukh A Mir1, Mahesh S Tirumkudulu1

  • 1IIT Bombay, Department of Chemical Engineering, IIT Bombay, Powai, Mumbai, 400076, Maharashtra, India.

Biosensors & Bioelectronics
|May 19, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a low-cost, compact flow cell for flow cytometry, enabling particle and cell analysis. This economical design is suitable for resource-constrained settings, offering a viable alternative to expensive conventional systems.

Keywords:
Flow cytometry biomedical diagnostics hydrodynamic focusing sheath flow assembly forward scatter side scatter

More Related Videos

Author Spotlight: Innovative Laser Techniques for Hoechst Staining to Analyze Side Population Cells
06:31

Author Spotlight: Innovative Laser Techniques for Hoechst Staining to Analyze Side Population Cells

Published on: August 23, 2024

1.6K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.2K

Related Experiment Videos

Last Updated: Sep 22, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

23.8K
Author Spotlight: Innovative Laser Techniques for Hoechst Staining to Analyze Side Population Cells
06:31

Author Spotlight: Innovative Laser Techniques for Hoechst Staining to Analyze Side Population Cells

Published on: August 23, 2024

1.6K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.2K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optical Physics

Background:

  • Flow cytometry is a key diagnostic tool for analyzing cells and particles.
  • Conventional flow cells are often expensive quartz-based systems, limiting accessibility.
  • Hydrodynamic focusing is crucial for precise particle stream confinement in flow cells.

Purpose of the Study:

  • To design and demonstrate a compact, economical, and biocompatible flow cell assembly.
  • To provide an affordable alternative for flow cytometry in resource-limited environments.
  • To validate the performance of the novel flow cell for particle characterization.

Main Methods:

  • Developed a flow cell using inexpensive capillaries and polymer fixtures for hydrodynamic focusing.
  • Investigated the relationship between sample core diameter, and sample and sheath flow rates.
  • Utilized forward and side light scattering for particle enumeration and characterization.

Main Results:

  • The novel flow cell successfully focused particle streams within a tight region.
  • Particle size distributions from light scattering showed excellent agreement with direct imaging.
  • The flow cell accurately sized white blood cells in human blood samples.

Conclusions:

  • The developed flow cell offers a cost-effective and efficient solution for flow cytometry.
  • This design enhances the accessibility of advanced cell analysis techniques.
  • The flow cell is suitable for biomedical diagnostics and particle characterization in diverse settings.