Process optimization of the polyhydroxybutyrate production in the cyanobacteria Synechocystis sp. and Synechococcus...

The effect of four parameters (acetate, NaCl, inorganic carbon and days in darkness) affecting the polyhydroxybutyrate (PHB) production were tested and optimized for Synechococcus sp. and Synechocystis sp. using a Box-Behnken design. The optimal conditions for Synechocystis sp. were found to be 1.2 g L^-1 of acetate, 4 gC L^-1 of NaHCO₃, 18 g L^-1 of NaCl and 0 days in darkness. For Synechococcus sp., equal acetate concentration and days in darkness, and lower inorganic carbon and NaCl concentrations than those for Synechocystis sp. were needed (0.05 g L^-1 inorganic carbon and 9 g L^-1 NaCl). Optimal conditions were scaled up to 3 L photobioreactors. Using Synechocystis sp., 5.6 %_dcw of PHB was obtained whether adding or not acetate. In opposition, a maximum of 26.1 %_dcw by using acetate was reached with Synechococcus sp. These results provide an easy method to optimize the cultivation conditions to enhance PHB production with cyanobacteria.