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Updated: Sep 22, 2025
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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
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Pramef12 enhances reprogramming into naïve iPS cells
Daiki Haraguchi1, Toshinobu Nakamura1,2,3
1Gaduate School of Bio-Science, Japan.
Biochemistry and Biophysics Reports
|May 20, 2022
Summary
Preferentially expressed antigen of melanoma family member 12 (Pramef12), found in oocytes, boosts induced pluripotent stem (iPS) cell generation. Pramef12 enhances reprogramming efficiency by activating the Wnt/β-catenin pathway.
Area of Science:
- Stem cell biology
- Epigenetics
- Developmental biology
Background:
- Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells using defined transcription factors (OKSM).
- Oocyte-derived factors may enhance reprogramming efficiency, as suggested by somatic cell nuclear transfer studies.
- Preferentially expressed antigen of melanoma family member 12 (Pramef12) is highly expressed in oocytes.
Purpose of the Study:
- To investigate the role of Pramef12 in enhancing induced pluripotent stem cell generation.
- To elucidate the molecular mechanisms by which Pramef12 influences reprogramming.
Main Methods:
- Overexpression of Pramef12 in mouse fibroblasts during OKSM-induced reprogramming.
- Analysis of pluripotency-associated gene expression (including Gtl2 in the Dlk1-Dio3 imprinted region).
- Assessment of metabolic gene expression (glycolysis and oxidative phosphorylation).
- Evaluation of mesenchymal-to-epithelial transition (MET).
- Measurement of β-catenin activation.
Main Results:
- Overexpression of Pramef12 significantly enhanced the efficiency of iPS cell derivation.
- Pramef12 promoted the expression of naïve pluripotency genes, Gtl2, glycolysis-related genes, and oxidative phosphorylation-related genes.
- Pramef12 facilitated mesenchymal-to-epithelial transition during iPS cell generation.
- Pramef12 strongly activated β-catenin signaling.
Conclusions:
- Pramef12 enhances OKSM-induced reprogramming of somatic cells into iPS cells.
- Pramef12-mediated enhancement of reprogramming is associated with activation of the Wnt/β-catenin pathway.
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