Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

450
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
450
CRISPR01:59

CRISPR

53.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.1K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.5K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

157
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
157
Homologous Recombination02:31

Homologous Recombination

53.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
53.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment of A Mouse Sepsis-Induced Immunosuppression Model By Intranasal Instillation of Bacteria.

Journal of visualized experiments : JoVE·2026
Same author

Effect of Various Mulberry Leaf Powders on the Quality of Artificial Diet for Domestic Silkworm, Bombyx mori.

Insects·2026
Same author

Targeting a conserved flagellin D1 epitope with 16G10 confers cross-serotype protection in murine <i>Pseudomonas aeruginosa</i> infection models.

Human vaccines & immunotherapeutics·2026
Same author

Beyond DNA editing: how Cas13 redefined programmable RNA manipulation and what still limits its therapeutic promise.

Nucleic acids research·2026
Same author

<i>OsRDH</i> modulates multiple grain quality traits and contributes to rice seed germination and root growth development.

Frontiers in plant science·2026
Same author

Centromere protein J governs reproductive development and fertility in the silkworm Bombyx mori.

Insect biochemistry and molecular biology·2026
Same journal

Smart biomaterials: From responsiveness to closed-loop sensing and feedback.

Trends in biotechnology·2026
Same journal

Bacterial spores as a modular platform for the production of amyloids for materials.

Trends in biotechnology·2026
Same journal

The oriGen case and Mexico's regulatory blind spots in genomic biobanking.

Trends in biotechnology·2026
Same journal

A caspase-3-activated protein expression system for apoptosis visualization and apoptosis-pyroptosis conversion to boost antitumor activity.

Trends in biotechnology·2026
Same journal

Over 4 months of ethylene production using solid-state photosynthetic cell factories.

Trends in biotechnology·2026
Same journal

Closing the nitrogen loop in groundwater with biohybrid technologies.

Trends in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Sep 22, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.5K

CRISPR-Cas-mediated diagnostics.

Luoxi Li1, Guanwang Shen2, Min Wu3

  • 1Biological Science Research Center, Southwest University, Chongqing 400716, China; Wound Trauma Medical Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China.

Trends in Biotechnology
|May 20, 2022
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas technology offers a revolutionary approach to molecular diagnostics, overcoming limitations of traditional PCR methods. This innovation promises sensitive, specific, and accessible nucleic acid detection for broader applications.

Keywords:
CRISPR-Cas systemCRISPR-Cas toolboxdisease detectionmolecular diagnosisoptimization application strategies

More Related Videos

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.8K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

570

Related Experiment Videos

Last Updated: Sep 22, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.5K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.8K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

570

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Traditional nucleic acid detection methods, primarily PCR, offer high sensitivity and specificity.
  • However, PCR-based methods face limitations including high costs, need for skilled personnel, and logistical challenges for field deployment.

Purpose of the Study:

  • To review recent advancements in CRISPR-Cas based molecular diagnostic technologies.
  • To highlight the transformative impact of CRISPR-Cas systems on the field of molecular diagnostics.

Main Methods:

  • Review of current literature on CRISPR-Cas applications in molecular diagnostics.
  • Analysis of novel CRISPR-based diagnostic platforms and their performance.

Main Results:

  • CRISPR-Cas technology presents a promising alternative for molecular diagnostics.
  • These systems offer potential for improved sensitivity, specificity, and ease of use compared to traditional methods.

Conclusions:

  • CRISPR-Cas based diagnostics are poised to revolutionize molecular detection.
  • These technologies address key limitations of existing methods, enabling wider accessibility and application.