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Updated: Sep 22, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Label-Free DNA Sequence Detection through FRET from a Fluorescent Polymer with Pyrene Excimer to SG
Chen Xu1, Ruyi Zhou1, Ruichen Zhang1
1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Abstract:
A label-free complex probe composed of a water-soluble fluorescent pyrene-functionalized polymer, ssDNA, and a nucleic acid stain (SG) is presented here, which can detect DNA sequence via FRET from pyrene excimer to SG. Complementary and one-base mismatched strands at nanomolar concentrations can be distinguished by the examination of the FRET fluorescence intensity of SG. This novel strategy for detecting DNA using the fluorescent pyrene-functionalized polymer not only affords a simple label-free method to detect nucleic acid sequence but also endows the detection with high sensitivity and selectivity, which may find wide applications for optical biosensing.
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