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Published on: October 25, 2017
Nanoslit Confined DNA at Low Ionic Strengths
Jinyong Lee1, Sulhwa Kim1, Huisu Jeong2
1Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, Republic of Korea.
Abstract:
We present nanoslit confined DNA conformations at very low ionic strengths and a theory to explain most measurements for single DNA molecule size under strong nanoslit confinement. Very low ionic strength conditions not only increase the DNA persistence length dramatically, but also cause DNA molecules to swell to the extent that the effective diameter of DNA becomes larger than the nanoslit height. By accounting for these effects, our results and theory provide a reasonable clue for a current controversy regarding the dependence of the DNA conformation on slit height (h), persistence length (p), and effective diameter (w).
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