Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 22, 2025

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
09:25

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury

Published on: December 31, 2017

9.6K

Imaging the Deep Spinal Cord Microvascular Structure and Function with High-Speed NIR-II Fluorescence Microscopy.

Hequn Zhang1,2, Liang Zhu1,2,3, Dave Schwinn Gao4

  • 1Interdisciplinary Institute of Neuroscience and Technology (ZIINT), Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310020, China.

Small Methods
|May 23, 2022
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiplexed optoacoustic tracking and magnetic actuation of labeled blood cells in living mice.

Science advances·2026
Same author

Two-photon imaging of excitatory and inhibitory neural response to infrared neural stimulation.

Neurophotonics·2024
Same author

Enhancing Total Optical Throughput of Microscopy with Deep Learning for Intravital Observation.

Small methods·2023
Same author

Single-microvessel occlusion produces lamina-specific microvascular flow vasodynamics and signs of neurodegenerative change.

Cell reports·2023
Same author

NIR-II fluorescence in vivo confocal microscopy with aggregation-induced emission dots.

Science bulletin·2023
Same author

Precision 1070 nm Ultrafast Laser-Induced Photothrombosis of Depth-Targeted Vessels In Vivo.

Small methods·2022

Near-infrared II (NIR-II) fluorescence microscopy enables deep spinal cord imaging up to 350 µm. This technique captures red blood cell velocity changes, offering new insights for spinal cord research and clinical applications.

Area of Science:

  • Neuroscience
  • Biomedical Imaging
  • Vascular Biology

Background:

  • The spinal cord (SC) is vital for processing sensory and autonomic signals.
  • High optical scattering in the SC limits in vivo imaging depth.
  • Existing techniques like multiphoton microscopy have slow imaging speeds, hindering dynamic vascular studies.

Purpose of the Study:

  • To demonstrate deep spinal cord vascular imaging using near-infrared II (NIR-II) fluorescence.
  • To assess red blood cell (RBC) dynamics in the SC vasculature in vivo.
  • To establish NIR-II as a viable spectral window for SC research.

Main Methods:

  • Utilized NIR-II fluorescence microscopy for deep SC imaging (up to 350 µm).
  • Labeled red blood cells with indocyanine green (ICG), a clinically approved NIR dye.
Keywords:
NIR-II microscopyRBC tracinghigh-speed imagingindocyanine greenspinal cord vasculature

More Related Videos

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

2.0K
In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
10:24

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

Published on: January 5, 2012

24.2K

Related Experiment Videos

Last Updated: Sep 22, 2025

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
09:25

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury

Published on: December 31, 2017

9.6K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

2.0K
In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
10:24

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

Published on: January 5, 2012

24.2K
  • Employed a fast NIR camera for high-speed (100 fps) imaging of RBC flow.

    • Main Results:

    • Achieved deep SC vascular structural imaging comparable to two-photon microscopy.
    • Successfully captured high-speed dynamics of RBC velocity changes during hind leg stimulation.
    • Demonstrated the feasibility of NIR-II imaging for SC vasculature.

    Conclusions:

    • NIR-II fluorescence microscopy offers a promising spectral window for deep spinal cord imaging.
    • This technique overcomes limitations of traditional methods for visualizing SC vasculature.
    • NIR-II microscopy holds significant potential for both clinical and basic science research on the spinal cord.