Precision 1070 nm Ultrafast Laser-Induced Photothrombosis of Depth-Targeted Vessels In Vivo

Area of Science:

• Neuroscience
• Biomedical Engineering
• Optical Imaging

Background:

• Cerebrovasculature is vital for brain health and disease.
• Existing in vivo vascular thrombosis methods face challenges in accuracy and depth-selectivity.
• Targeting single cerebral cortex vessels requires advanced techniques.

Purpose of the Study:

• To evaluate Rose Bengal (RB)-induced photothrombosis using ultrafast lasers.
• To develop a precise and reproducible method for inducing vascular occlusion.
• To enhance the study of cerebrovascular function with high spatial-temporal resolution.

Main Methods:

• Evaluation of RB-induced photothrombosis with 720-1070 nm ultrafast lasers in a raster scan.
• Development of precision ultrafast laser-induced photothrombosis (PLP) using a 1070 nm ultrafast laser with a spiral scan.
• Integration of PLP with two-photon microscopy for real-time imaging.

Main Results:

• Demonstrated feasibility and effects of RB-induced photothrombosis.
• Achieved highly precise and fast occlusion induction of various vessel types, sizes, and depths using PLP.
• PLP enhances the precision and power of photothrombosis protocols.

Conclusions:

• PLP offers a practical, precise, and depth-selected single-vessel photothrombosis technology.
• The method utilizes commercially available optical equipment.
• PLP is crucial for exploring brain vascular function with high spatial-temporal resolution.