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Related Experiment Video

Updated: Sep 22, 2025

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

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Published on: June 30, 2018

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A protocol to evaluate immunoglobulin deposits in mouse glomeruli.

Sonia B Nambadan1, Maurice Stanley1,2, Yaoyuan Zhang1,2

  • 1Department of Immunity, Inflammation and Infection, The John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia.

STAR Protocols
|May 23, 2022
PubMed
Summary

This study presents a protocol for detecting immune deposits like immunoglobulin and complement in mouse kidneys. This method aids in diagnosing glomerulonephritis and understanding kidney injury mechanisms.

Keywords:
ImmunologyMicroscopyModel Organisms

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Area of Science:

  • Nephrology
  • Immunopathology
  • Histology

Background:

  • Glomerulonephritis is characterized by immune deposits in the kidneys.
  • Detecting immunoglobulin (IgG, IgA, IgM) and complement (C3) deposits aids diagnosis and understanding of nephritis.
  • Accurate detection methods are crucial for studying kidney injury.

Purpose of the Study:

  • To describe a detailed protocol for detecting immune deposits in biological specimens, specifically mouse kidneys.
  • To provide a method for characterizing and quantifying immune deposits contributing to kidney injury.
  • To support the diagnostic and pathological investigation of glomerulonephritis.

Main Methods:

  • Tissue isolation and processing of mouse kidneys.
  • Immunostaining techniques for detecting specific immune components.
  • Fluorescence microscopy for visualization and quantification of deposits.

Main Results:

  • The protocol enables the detection of immunoglobulin and complement components within kidney tissues.
  • Characterization and quantification of immune deposits are achievable using this method.
  • The described technique facilitates the study of pathological processes in glomerulonephritis.

Conclusions:

  • The developed protocol is effective for detecting immune deposits in mouse kidneys.
  • This method aids in the diagnosis and pathological assessment of glomerulonephritis.
  • The protocol offers a valuable tool for researchers investigating kidney injury.