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Transfer RNA Synthesis02:35

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Related Experiment Video

Updated: Sep 22, 2025

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
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PltRNAdb: Plant transfer RNA database.

Morad M Mokhtar1, Achraf El Allali1

  • 1African Genome Center, University Mohammed VI Polytechnic, Benguerir, Morocco.

Plos One
|May 23, 2022
PubMed
Summary
This summary is machine-generated.

Researchers identified over 330,000 transfer RNA (tRNA) genes across 256 plant species, creating a comprehensive database. This resource, PltRNAdb, aids in understanding tRNA functions in plant genomes.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Transfer RNAs (tRNAs) are crucial non-coding RNAs for protein synthesis in all organisms.
  • The rapid increase in sequenced plant genomes offers unprecedented opportunities for large-scale tRNA studies.

Purpose of the Study:

  • To identify and catalog tRNA genes across a wide range of plant species.
  • To establish a centralized, accessible database for plant tRNA genes.

Main Methods:

  • Analysis of whole-genome sequences from 256 plant species, totaling 8,768,261 scaffolds/chromosomes and 229,093 gigabase pairs.
  • Identification of nuclear, chloroplast, and mitochondrial tRNA genes using computational approaches.
  • Development of the Plant tRNA database (PltRNAdb) for data accessibility and analysis.

Main Results:

  • Identification of 331,242 nuclear, 3,216 chloroplast, and 1,467 mitochondrial tRNA genes.
  • Characterization of nuclear tRNA genes, including those for standard amino acids, suppressor tRNAs, unknown isotypes, pseudogenes, and those with introns.
  • Creation of PltRNAdb, a web-accessible resource for searching, browsing, visualizing, and downloading plant tRNA gene data.

Conclusions:

  • The comprehensive analysis provides a significant expansion of known plant tRNA genes.
  • PltRNAdb serves as a valuable resource for plant molecular biology and genomics research.
  • This resource facilitates further investigation into the regulatory roles of tRNAs in plant biology.