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Updated: Sep 22, 2025

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

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Published on: January 3, 2016

A sensitive and compact optical detector based on digital lock-in amplification.

Andrew J Harvie¹, Surendra K Yadav¹, John C de Mello¹

¹Department of Chemistry, NTNU, Trondheim, Norway.

|May 24, 2022

Summary

This summary is machine-generated.

Researchers developed a sensitive optical detector using a light-emitting diode and microcontroller. This affordable device achieves low detection limits for fluorescence assays, adaptable for various biomolecules.

Keywords:

3D printing Bioassays Digital signal processing Fluorescence Lock-in Open hardware

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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

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Published on: June 13, 2010

Area of Science:

Analytical Chemistry
Biomedical Engineering
Spectroscopy

Background:

Accurate and sensitive detection of biomolecules is crucial for diagnostics.
Existing methods can be expensive or require complex instrumentation.
Development of cost-effective, versatile optical detection systems is needed.

Purpose of the Study:

To design and characterize a sensitive, low-cost, lock-in-based optical detector.
To demonstrate its utility for both absorption and fluorescence measurements.
To adapt the system for biomolecule quantification using enzymatic assays.

Main Methods:

Construction of a detector using a light-emitting diode, filters, photodetector, analogue components, and a microcontroller.
Implementation of a lock-in detection technique for enhanced sensitivity.
Application to a cholesterol assay involving Amplex Red and resorufin fluorescence.

Main Results:

The optical detector demonstrated high sensitivity, with a detection limit of < 200 nM for cholesterol assay.
Resorufin fluorescence was detectable down to 20 nM.
The system is suitable for both cuvette and flow-cell measurements (<100 μl).

Conclusions:

A cost-effective and sensitive optical detector was successfully developed.
The system is versatile and can be readily adapted for detecting various biomolecules.
This technology offers a promising platform for low-cost biochemical analysis.