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Updated: Jul 14, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

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Methods for Assessing Circadian Rhythms and Cell Cycle in Intestinal Enteroids.

Miri Park¹, Yuhui Cao¹, Christian I Hong²

¹Department of Pharmacology and Systems Physiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 24, 2022

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This study presents methods to assess circadian rhythms in patient-derived gut organoids. These techniques can reveal patient-specific circadian clock dynamics for personalized medicine applications.

Area of Science:

Chronobiology
Gastroenterology
Personalized Medicine

Background:

Circadian clocks regulate vital biological processes, and their disruption is linked to various diseases.
Understanding patient-specific circadian rhythms is crucial for personalized medicine and optimized drug delivery.
Patient-derived 3D enteroids offer a model to study human-specific circadian biology.

Purpose of the Study:

To introduce and validate methods for assessing circadian rhythms and cell cycle dynamics in intestinal enteroids.
To enable the evaluation of patient-specific circadian clock function in vitro.
To support the development of personalized medicine strategies for circadian-related disorders.

Main Methods:

Time-course sample collection from mouse and human intestinal enteroids.

Keywords:

Bioluminescence assay Cell cycle Circadian rhythm Immunofluorescence Intestinal enteroids Time course sampling

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Last Updated: Jul 14, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

Quantification of Proliferative and Dead Cells in Enteroids

Quantification of Proliferative and Dead Cells in Enteroids

Published on: January 31, 2020

Monitoring Circadian Oscillations with a Bioluminescence Reporter in Organoids

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Published on: February 16, 2024

Application of immunofluorescence and live-cell confocal microscopy.

Utilizing bioluminescence assays to monitor circadian rhythms and cell division.

Main Results:

Established procedures for assessing circadian rhythms and cell cycle dynamics in enteroids.
Demonstrated the utility of enteroids as a model for studying patient-specific circadian biology.
Provided a framework for evaluating circadian clock-gated cell division in intestinal organoids.

Conclusions:

Enteroid models provide a powerful platform for studying human circadian rhythms.
The presented methods facilitate the assessment of patient-specific circadian clock function.
These advancements hold promise for personalized medicine in circadian-related diseases.