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Updated: Sep 22, 2025
10:04
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
Published on: July 6, 2017
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Chromatin Immunoprecipitation and Circadian Rhythms
Kenichiro Kinouchi1, Kazutoshi Miyashita2, Hiroshi Itoh2
1Division of Endocrinology, Metabolism, and Nephrology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan. ken-k@keio.jp.
Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2022
Summary
Organisms adapt to daily cycles via rhythmic gene expression controlled by the core clock machinery. Chromatin immunoprecipitation (ChIP) reveals how this machinery regulates the circadian epigenome.
Area of Science:
- Molecular Biology
- Chronobiology
- Epigenetics
Background:
- Organisms display daily physiological and behavioral adaptations to environmental day-night cycles.
- These adaptations involve rhythmic gene expression orchestrated by a core molecular clock.
- The circadian system ensures homeostatic balance through these daily oscillations.
Purpose of the Study:
- To summarize the methodology of chromatin immunoprecipitation (ChIP) experiments.
- To elucidate the role of ChIP in understanding circadian rhythm research.
- To highlight how ChIP contributes to deciphering the regulation of rhythmic gene expression.
Main Methods:
- Chromatin immunoprecipitation (ChIP) is detailed as a key technique.
- The method involves analyzing the interaction of transcription factors and chromatin modifications.
- High-throughput sequencing technologies are integrated for broader analysis.
Main Results:
- ChIP reveals how core clock components and transcription factors regulate the circadian epigenome.
- Cyclic alterations in chromatin structure and molecular functions are identified.
- This provides insights into the mechanisms driving rhythmic gene expression.
Conclusions:
- Chromatin immunoprecipitation is crucial for dissecting the molecular mechanisms of circadian rhythms.
- Understanding the circadian epigenome is essential for comprehending daily adaptations.
- Advancements in sequencing technology enhance circadian research capabilities.
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