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PEGDA microencapsulated allogeneic islets reverse canine diabetes without immunosuppression.
Stephen Harrington1, Francis Karanu1, Karthik Ramachandran1
1Likarda, LLC, Kansas City, Missouri, United States of America.
Plos One
|May 25, 2022
Summary
This study shows polyethylene glycol diacrylate (PEGDA) microencapsulated islets can reverse diabetes in dogs without immunosuppression, leading to insulin independence. This offers a promising new approach for islet transplantation.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Transplantation Immunology
Background:
- Achieving islet transplant success without systemic immunosuppression remains a significant challenge.
- This study explores polyethylene glycol diacrylate (PEGDA) microencapsulation as a potential solution for islet protection.
Purpose of the Study:
- To evaluate the biocompatibility and safety of PEGDA microencapsulated canine islets in healthy dogs.
- To determine the efficacy of these microencapsulated islets in reversing diabetes in diabetic dogs.
Main Methods:
- Canine islets were characterized and encapsulated in PEGDA microspheres.
- Islets were transplanted intraperitoneally into healthy and diabetic dogs.
- Biocompatibility, safety, and glycemic control were monitored.
Main Results:
- No adverse events or hypoglycemia were observed in healthy dogs receiving up to 1.7 M cells/kg.
- Diabetic dogs showed a 50-100% reduction in insulin requirements, with some achieving insulin independence.
- Microencapsulated islets attached to the omentum, and abnormal blood chemistry values normalized post-transplant.
Conclusions:
- PEGDA microencapsulated canine islets effectively reversed diabetes signs in dogs without requiring systemic immunosuppression.
- This approach shows potential for achieving insulin independence or significantly reduced insulin needs in islet transplant recipients.
