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Related Experiment Videos

Creatine kinase isoforms following isometric exercise.

P M Clarkson, F S Apple, W C Byrnes

    Muscle & Nerve
    |January 1, 1987
    PubMed
    Summary
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    Analyzing creatine kinase (CK) isoforms in serum offers an earlier detection of skeletal muscle damage than total CK or soreness. This method aids in tracking muscle injury and recovery timelines.

    Area of Science:

    • Exercise Physiology
    • Biochemistry
    • Sports Medicine

    Background:

    • Skeletal muscle damage from exercise is common.
    • Assessing muscle damage typically involves measuring creatine kinase (CK) activity and subjective soreness.
    • Understanding the kinetics of CK release and transformation in serum is crucial.

    Purpose of the Study:

    • To evaluate creatine kinase (CK) activity, CK MM isoforms, and muscle soreness after exercise-induced skeletal muscle damage.
    • To determine if CK isoforms provide an earlier indicator of muscle damage compared to total CK or soreness.
    • To assess the utility of CK isoforms in monitoring the time course of muscle damage and repair.

    Main Methods:

    • Eight college-age subjects performed 40 maximal isometric knee extensor contractions.

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  • Serum samples and soreness ratings were collected pre-exercise and at 2, 6, 18, and 24 hours post-exercise.
  • Creatine kinase (CK) MM1 and CK MM3 isoforms were analyzed using flatbed isoelectric focusing (IEF).

    • Main Results:

    • Serum CK activity significantly increased at 6 hours post-exercise, remaining elevated at 24 hours.
    • The CK MM1 isoform, indicative of newly released CK, increased significantly at 2 hours, peaked at 6 hours, and then returned towards baseline.
    • Muscle soreness ratings did not significantly increase until 18 hours post-exercise.

    Conclusions:

    • Analysis of CK isoforms in serum can serve as an earlier indicator of skeletal muscle damage than total CK activity or perceived soreness.
    • CK isoform analysis may be valuable for tracking the temporal progression of skeletal muscle damage and subsequent repair processes.
    • This approach enhances the understanding of muscle injury markers following strenuous exercise.