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Updated: Sep 22, 2025
A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
Published on: March 23, 2015
Production of Recombinant Proteins in Transgenic Tobacco Plants
Tim H Szeto1, Pascal M W Drake2, Audrey Y-H Teh1
1Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Abstract:
Nicotiana tabacum (the tobacco plant ) has numerous advantages for molecular farming, including rapid growth, large biomass and the possibility of both cross- and self-fertilization. In addition, genetic transformation and tissue culture protocols for regeneration of transgenic plants are well-established. Here, we describe the production of transgenic tobacco using Agrobacterium tumefaciens and the analysis of recombinant proteins, either in crude plant extracts or after purification, by enzyme-linked immunosorbent assays, sodium dodecyl sulfate polyacrylamide gel electrophoresis with western blotting and surface plasmon resonance.
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