Ultrasensitive Molecular Detection at Subpicomolar Concentrations by the Diffraction Pattern Imaging with Plasmonic...

Compact and cost-effective optical devices for highly sensitive detection of trace molecules are significant in many applications, including healthcare, pollutant monitoring and explosive detection. Nanophotonic metasurface-based sensors have been intensively attracting attentions for molecular detection. However, conventional methods often involve spectroscopic characterizations that require bulky, expensive and sophisticated spectrometers. Here, a novel ultrasensitive sensor of plasmonic metasurfaces is designed and fabricated for the detection of trace molecules. The sensor features a convex holographic grating, of which the first-order diffraction pattern of a disposable metasurface is recorded by a monochrome camera.The diffraction pattern changes with the molecules attached to the metasurface, realizing label-free and spectrometer-free molecular detection by imaging and analyzing of the diffraction pattern. By integrating the sensor with a microfluidic setup, the quantitative characterization of rabbit anti-human Immunoglobulin G (IgG) and human IgG biomolecular interactions is demonstrated with an excellent limit of detection (LOD) of 0.6 pm. Moreover, both the metasurface and holographic grating are obtained through vacuum-free solution-processed fabrications, minimizing the manufacturing cost of the sensor. A prototype of the imaging-based sensor, consisting of a white light-emitting diode (LED) and a consumer-level imaging sensor is achieved to demonstrate the potential for on-site detection.